Priming with CuO Nanoparticles and Ultrasound Enhanced Antioxidant Potential and Total Taxol in the Cell Suspension Culture of Corylus avellana L.

Document Type : Research Article

Authors

1 Department of Horticulture Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Department of Horticulture and Landscape, Faculty of Agriculture, University of Zabol, Zabol, Iran

3 MSc in Horticultural Sciences, Sari Agricultural Sciences and Natural Resources University, sari, Iran

10.22034/jon.2021.1925927.1109

Abstract

The main superiority of taxol production in hazelnuts compared to the yew tree is that hazelnut is broadly accessible, grows much faster in nature, and is easier to establish in the in vitro systems. The aim of this experiment was to improve taxol production and evaluate other characteristics of cell suspension culture treated with 4 concentrations (0, 2, 4, 6 mg L-1) of copper oxide nanoparticles (CuO NPs), ultrasonication (30 and 60 seconds) in two sampling times (9 and 18 days after treatment). Interaction of all three factors was significant on fresh weight (FW), protein content, H2O2 generation, and taxol content. The highest fresh and dry weights (DW) and protein content were obtained from non-application treatments of CuO NPs. The content of anthocyanins, proline, antioxidant activity, and catalase and peroxidase enzymes increased with the application of silver nitrate nanoparticles. Comparing means for interaction effects showed that H2O2 and taxol content have nearly similar trend, indicating the stimulation of taxol production pathway by reactive oxygen species (ROS). Ultrasonication (US) enhanced most of the traits studied in this experiment and stimulated the antioxidant system without negatively affecting on cellular growth. The antioxidant response and taxol content evoked by stress treatments i.e. US and CuO NPs is partially relieved by increasing the duration after treatment (the 18th day after treatment).

Keywords


Aebi H, Bergmeyer HU (1983) Methods of Enzymatic Analysis. In Catalase; Verlag Chemie: Weinheim, Germany, Volume 3, pp. 273–277.
Aslani Aslamarz AA, Vahdati K, Hasani D, Rahemi M, Leslie CA (2011) Cold hardiness and its relationship with proline content in Persian walnut. European Journal of Horticultural Sciences. 76 (3), 84–90.
Bates LS, Waldern RP, Teare I (1973) Rapid determination of free proline for water stress studies. Plant Soil. 39(1), 205-207.
Bertini L, Focaracci F, Proietti S, Papetti P, Caruso C (2019) Physiological response of Posidonia oceanica to heavy metal pollution along the Tyrrhenian coast. Functional Plant Biology. 46, 933–941.
Bestoso F, Ottaggio L, Armirotti A, Balbi A, Damonte G, Degan P, Mazzei M, Cavalli F, Ledda B, Miele M (2006) In vitro cell cultures obtained from different explants of Corylus avellana produce taxol and taxanes. BMC Biotechnology. 6, 1-11.
Chung IM, Rajakumar G, Subramanian U, Venkidasamy B, Thiruvengadam M (2019) Impact of copper oxide nanoparticles on enhancement of bioactive compounds using cell suspension cultures of Gymnema sylvestre (Retz.) R. Br. Applied Sciences. 9(10), 1-17.‏
Chung IM, Rekha K, Rajakumar G, Thiruvengadam M (2018) Elicitation of silver nanoparticles enhanced the secondary metabolites and pharmacological activities in cell suspension cultures of bitter gourd. 3 Biotech. 8 (10), 1-12.
Cusido RM, Onrubia M, Sabater-Jara AB, Moyano E, Bonfill M, Goossens A, Pedreno MA, Palazon J (2014) A rational approach to improving the biotechnological production of taxanes in plant cell cultures of Taxus spp. Biotechnology Advances. 32(6), 1157-1167.
Fakruddin MD, Hossain Z, Afroz, H (2012) Prospects and applications of nanobiotechnology: A medical perspective. Journal of Nanobiotechnolog. 10, 1–8.
Farrokhzad Y, Pourmand S, Rezaei A, Sharafi Y (2016) Effects of ultrasound, tryptophan and proline on embryogenesis and regeneration of grape (Vitis vinifera L.). Azarian Journal of Agriculture. 3(5), 112-118.‏
Farrokhzad Y, Rezaei A (2020) Aluminum elicitation improves antioxidant potential and taxol production in hazelnut (Corylus avellana L.) cell suspension culture. Agriculturae Conspectus Scientificus. 85(3), 229-236.‏
Feigl G, Kumar D, Lehotai N, Tugyi N, Molnár Á, Ördög A, Szepesi Á, Gémes K, Laskay G, Erdei L (2013) Physiological and morphological responses of the root system of Indian mustard (Brassica juncea L. Czern.) and rapeseed (Brassica napus L.) to copper stress. Ecotoxicology and Environmental Safety. 94, 179–189.
Fett-neto AG, Melanson S, Sakata F, DiCosmo J (1993) Improved growth and Taxol yield in developing calli of Taxus cuspidata by medium composition modification. Biotechnology. 11, 1007–1012
Gallego A, Malik S, Yousefzadi M, Makhzoum A, Tremouillaux-Guiller J, Bonfill M (2017) Taxol from Corylus avellana: paving the way for a new source of this anti-cancer drug. Plant Cell, Tissue and Organ Culture (PCTOC). 129(1), 1-16.
Gatahi DM, Wanyika HN, Kavoo A, Kihurani A, Ateka EM (2016) Enhancement of bacterial wilt resistance and rhizosphere health in tomato using bionanocomposites. International Journal of Horticultural Science and Technology. 3(2), 129-144.
Itokawa H (2003) Taxoide occurring in the genus Taxus: 35-78. In: Itokawa H, Lee KH, (Eds.) Taxus: The Genus Taxus. Taylor & Francis Press. pp. 427.
Jariteh M, Ebrahimzadeh H, Vahdati K, Niknam V and Mirmasoumi M (2011) Antioxidant enzymes activities during secondary somatic embryogenesis in Persian walnut (Juglans regia L.). African Journal of Biotechnology 10(20), 4093-4099.
Jariteh M, Ebrahimzadeh H, Niknam V, Mirmasoumi M and Vahdati K (2015) Developmental changes of protein, proline and some antioxidant enzymes activities in somatic and zygotic embryos of Persian walnut (Juglans regia L.). Plant, Cell, Tissue and Organ Culture. 122, 101–115.
Kar M, Mishra D (1976) Catalase, peroxidase, polyphenol oxidase activities during rice leaf senescence. Plant Physiology. 57, 315–319.
Lotfi N, Vahdati K, Kholdebarin B and Amiri R (2010) Drought-induced accumulation of sugars and proline in radicle and plumule of tolerant walnut varieties during germination phase. Acta Horticulturae. 861, 289-296.
Murakami M, Ae N (2009) Potential for phytoextraction of copper, lead, and zinc by rice (Oryza sativa L.), soybean (Glycine max (L.) Merr.), and maize (Zea mays L.). Journal of Hazardous Materials. 162, 1185–1192.
Murashige T, Skoog F (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum. 15(3), 473-497.‏
Nasibi F, Manouchehri Kalantari K, Manzari Tavakoli Z (2020) Effects of Hydrogen Sulfide on Cold-Induced Oxidative Damage in Cucumis sativus L. International Journal of Horticultural Science and Technology. 7(3), 199-211
Ramirez-Estrada K, Vidal-Limon H, Hidalgo D, Moyano E, Golenioswki M, Cusidó RM, Palazon J (2016) Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecule. 21, 1-24.
Rezaei A, Ghanati F, Behmanesh M, Mokhtari-Dizaji M (2011) Ultrasound potentiated salicylic acid-induced physiological effects and production of Taxol in hazelnut (Corylus avellana L.) cell culture. Ultrasound in Medicine and Biology. 37(11), 1938–1947.
Tabata H (2004) Paclitaxel production by plant cell culture technology. Advances in Biochemical Engineering/Biotechnology. 87, 1–23
Wagner GJ (1979) Content and vacuole/extra-vacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplasts. Plant Physiology. 64(1), 88-93.
Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances. 23, 283–333