Physiological Responses of Some Rootstocks and Interspecific Hybrids of Pistachio to Cold Stress under Greenhouse Conditions

Document Type: Research Article


1 Department of Horticulture Science, University of Hormozgan, Hormozgan, Iran

2 Department of Horticulture, Education and Extention Institute, AREEO, Tehran, Iran

3 Pistachio Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran


Cold is an environmental factor limiting the growth and yield of agricultural crops. To find cold-tolerant pistachio rootstocks, a factorial experiment was conducted at Pistachio Research Center (PRC), Rafsanjan, Iran during 2017-2018. The factors included six rootstocks (Badami Zarand, Ghazvini, Sarakhs, Integerrima × Badami Zarand, Integerrima × Ghazvini and Integerrima × Sarakhs) and four thermal treatments (4, 0, -2, and -4°C) with three iterations. Seedlings in 6-8 leaf stage were placed in thermal treatments for two hours. Ionic leakage, pH of leaked solution, chlorophyll fluorescence, photosynthesis efficiency index, chlorophyll (a, b, total) and carotenoid contents were the traits assessed in this study. Based on the results, a decrease in temperature made an increase in ionic leakage rate, conversely making decreases in pH of the leaked solution, photosynthetic pigments contents, Fv/Fm and PI index. In addition, the highest photosynthetic pigments, pH of leaked solution, Fv/Fm and PI index were observed in Ghazvini and Integerrima × Ghazvini rootstocks. Meanwhile, the highest ionic leakage was observed in Integerrima × Badami Zarand. In aggregate, Ghazvini and Integerrima × Ghazvini were identified as the most cold-tolerant rootstocks, while Integerrima × Badami Zarand was the most cold-sensitive rootstock.


Afrousheh M, Hokmabadi H, Arab H, Tajabadipour A (2018) Evaluation of frost damage tolerance in some pistachio seedling rootstocks. Journal of Nuts. 9(1), 77-83.

Afshari H, Zahedi R, Parvaneh T, Zadehbagheri M (2014) Effect of salicylic acid on proline, soluble carbohydrate and ion leakage of two apricot varieties under cold stress. Journal of Agricultural Crops Production (Crops Improvement). 16(1), 127-138. [In Persian].

Ahmadi A, Jabbari F, Ehsanzadeh P (2009) Introduction to plant physiology. Tehran University Press (translated). pp, 651. 300-302. [In Persian].

Alipour, H., 2018. Photosynthesis properties and ion homeostasis of different pistachio cultivar seedlings in response to salinity stress. International Journal of Horticultural Science and Technology, 5(1), 19-29.

Aslani Aslamarz A, Vahdati K, Hasani D, Rahemi M, Leslie CA (2010) Supercooling and cold hardiness in the acclimated and deacclimated buds and stems of Persian walnut cultivars and genotypes. HortScience. 45 (11), 1-6.

Azzarello E, Mugnai S, Pandolfi C, Masi E, Marone E, Mancuso S (2009) Comparing image (fractal analysis) and electrochemical (impedance spectroscopy and electrolyte leakage) techniques for the assessment of the freezing tolerance in olive. Trees. 23, 159-167.

Baghbanha M, Fotouhi Ghazvini R, Hatamzadeh A, Heidari M (2007) Effect of salicylic acid on freezing tolerance of Mexican lime seedlings (Citrus aurantifolia L). Iranian Journal of Horticultural Science and Technology. 8(3), 185-198. [In Persian].

Baker NR (1991) A possible role for photosystem II in environmental perturbation of photosynthesis. Physiologia. Plantrum. 81, 563-570.

Barranco D, Ruiz N, Gómez-del Campo M (2005) Frost tolerance of eight olive cultivars. Hort Science. 40(3), 558-560.

Bartolozzi F and Fontanazza G (1999) Assessment of frost tolerance in olive (Olea europaea L.). Scientia Horticulturae. 81(3), 309-319.

Berova M, Zlatev Z, Stoeva N (2002) Effect of paclobutrazol on wheat seedlings under low temperature stress. Bulg. Journal Plant Physiology. 28, 75-84.

Bertin P, Kinet JM, Bouharmont Y (1996) Evaluation of chilling sensitivity in different rice varieties. Relationship between screening procedures applied during germination and vegetative growth. Euphytica. 89(2), 201-210.

Ershadi A and Taheri S (2013) Investigation of the effect of salicylic acid on spring frost in grapevine (Vitis vinifera). Journal of Agricultural Research. 15(2), 135-146. [In Persian].

FAO (2017) Food and Agriculture Organization of the United Nation (FAO),

Hakam N, Khanizadeh S, R. De Ell J, Richer C (2000) Assessing chilling tolerance in roses using chlorophyll fluorescence. HortScience. 35, 184-186.

Hasheminasab H, Afrousheh M (2018) Introduction of pistachio rootstocks and cultivars of America and their comparison with Iranian conditions. Horticultural Research Institute. pp, 62. 33-35. [In Persian].

Hokmabadi H, Rezaee L, Mohammadi Moghaddam M, Mortazavi A, Serfi H, Ghorbani A, Avazabadian A, Reziabadi H (2016) Investigation of cold resistance in three commercial cultivars of Damghan pistachio and three major cultivars of pistachio through ion leakage parameters. The final report of the research project of Iran Pistachio Research Institute. Damghan Pistachio Research Station. 72 P. [In Persian].

Imani A, Barzegar K, Piripireivatlou S (2011) Relationship between frost injury and ion leakage as an indicator of cold hardiness in 60 almond selections. Journal of Nuts. 2(1), 22-26.

Jafari SR, Manuchehri Kalantari Kh, Turkzadeh M (2006) The evaluation of paclobutrazol effects on increase cold hardiness in tomato seedlings (lycopersicum esculentum L.). Journal of Biology. 19(3), 290-298. [In Persian].

Keshavarz H and Modaress sanavi SAM (2014) Effect of salicylic acid on chlorophyll, some growth characteristics and yield of two canola varieties. Crop production publication. 7(4), 161-178. [In Persian].

Khorram A, Rabiee A, Imani A, Mortazavi SN (2011) Relationship between physiological indices and freezing injury in some almond cultivars at different phenophases of flower bud development. Journal of Horticultural Science. 12(1), 65-76. [In Persian].

Lahijanian S, Mobli M, Baninasab B, Etemadi N (2012) Investigation of cold resistance of different Eucalyptus genotypes by measuring chlorophyll fluorescence. First National Conference on Non-Biological Plant Stress. 10-11 November, Isfahan University of Technology. [In Persian].

Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions. 11, 591- 592.

Liu X and Huang B (2002) Cytokinine effects on creeping bent grass response to heat stress. Crop Science. 42, 466-472.

Lu JX, Jiang HY, Li W (2012) Effects of low temperature stress on the cold resistance of rootstock and branch of wine grapes. Journal of Fruit Science. 29(6), 1040-1046.

Mehata P, Jajoo A, Mathur S, Bharti S (2010) Chlorophyll a fluorescence study revealing effects of high salt stress on photosystem II in wheat leaves. Plant Physiology and Biochemistry. 48(1), 16-20.

Mirmohammadi Meybodi AM, Tarkesh Isfahani S (2004) Breeding and physiological aspects of cold and freezing stress in crops. Golbon Press. pp, 312. 27-31. [In Persian].

Moshtaghi EA, Shahsavar AR, Taslimpour MR (2009) Ionic leakage as indicators of cold hardiness in olive (Olea europaea L.). World Applied Science Journal. 7, 1308-1310.

Niyogi KK (1999). Photoprotection revisited: genetic and molecular approaches. Annual review of plant biology. 50(1), 333-359.

Palonen P (1999) Relationship of seasonal changes in carbohydrates and cold hardiness in canes and buds of three red raspberry cultivars. Journal of the American Society for Horticultural Science. 124(5), 509-513.

Percival GC, Henderson A (2003) An assessment of the freezing tolerance of urban trees using chlorophyll fluorescence. Journal of Horticultural Science and Biotechnology. 78(2), 254–260.

Porra RJ (2002) The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynthesis Research. 73, 149-156.

Roháček K, Soukupová J, Barták M (2008) Chlorophyll fluorescence: a wonderful tool to study plant physiology and plant stress. Plant Cell Compartments-Selected Topics. Research Signpost, Kerala, India. 41-104.

Sairam RK (1994) Effect of moisture stress on physiological activities of two contrasting wheat genotypes. Indian Journal of Experimental Biology. 32, 594-597.

Sakai A, Larcher W (1987) Frost Survival of Plants: Responses and Adaptation to Freezing Stress. Springer-Verlag.

Shamshiri MH, Hasani MR (2015) Synergistic accumulative effects between exogenous salicylic acid and arbuscular mycorrhizal fungus in pistachio (Pistacia vera cv. Abareqi) seedlings under drought stress. International Journal of Horticultural Science and Technology. 2(2), 151-160.

Simkeshzadeh N, Mobli M, Etemadi N, Banienasab b (2010) Evaluation of cold resistance in some olive cultivars by measuring chlorophyll fluorescence and appearance damages. Journal of Horticultural Science. 24(2), 163-169. [In Persian].

Sohrabi N, Hokmabadi H, Tajabadipour A (2009) Chilling injury physiology in pistachio trees. Extension Magazine, Iran Pistachio Research Institute. pp.35. [In Persian].

Strasser RJ, Srivastava A, Tsimilli-Michael M (2000) The fluorescence transient as a tool to characterize and screen photosynthetic samples. Probing photosynthesis: Mechanisms, Regulation and Adaptation. 94, 445-483.

Strauss AJ, Kruger GHJ, Strasser RJ, Van Heerden PDR (2006) Ranking of dark chilling tolerance in soybean genotypes probed by the chlorophyll a fluorescence transient O-J-I-P. Environmental and Experimental Botany. 56, 147-157.

Tajabadipour A, Fattahi Moghadam MR, Zamani Z, Nasibi F, Hokmabadi H (2018) Evaluation of physiological and biochemical changes of pistachio (Pistacia vera L. cv. Ahmad-Aghaii) on cold tolerant and sensitive rootstocks under freezing stress conditions. Journal of Horticultural Science. 32(3), 471-484. [In Persian].

Tajvar Y, Fotouhi Ghazvini R, Hamidoghli Y, Hassan Sajedi R (2011) Physiological and biochemical responses of page mandarin on citrange rootstock to low temperature stress. Journal of Plant Biology. 9, 1-12. [In Persian].

Terzaghi WB, Fork DC, Berry JA, Field CB (1989) Low and high temperature limits PSII. A survey using trans-parinaric acid, delayed light emission, and F0 chlorophyll fluorescence. Plant Physiology. 91, 1494-1500.

Tignor ME, Davies FS, Sherman WB (1998) Freezing tolerance and growth characteristics of USDA intergeneric citrus hybrids US 119 and selection 17-11. Hort Science. 33(4), 744–748.