Reactions of Various Cultivars of Almond to Toxin-Producing Aspergillus flavus Isolates

Document Type : Research Article


1 Instructor at the Agricultural Jihad Training Center of the Holy Prophet (PBUH), Damghan, Iran

2 Department of Agriculture, Damghan Branch, Islamic Azad University, Damghan, Iran

3 Faculty Member of the Pistachio Research Station, Damghan


Samples were collected from different regions of two provinces, Markazi and Semnan, their contamination by Aspergillus flavus was examined. The results of A. flavus colony counts in different samples of almond showed that among 20 experimented samples, 11 samples were contaminated by fungus; therefore, 8 cultivars of almond, Shahroud 6, Shahroud 21, Shahroud 12, Shahroud 8, Shahroud 17, Rabie, Mengha, and Sangi Shireen, were collected in order to evaluate their sensitivity to fungal colonization and the sporulation rate of fungus on them. The results of statistical analysis showed that on the third, fifth, and seventh days, Shahroud 12 was the least resistant cultivar [average pollution (99.5%)], whereas Shahroud 6 was the most resistant [average contamination (7.36%)] at a level of 5 % for growth of A. flavus after 7 days. Experiments related to the testa of almond show that the testa can be a barrier against penetration of fungus into the inner part of the almond, decreaseing fungal growth, and thus reducing the weight of mycelium and sporulation in almonds.


Afshari H, Abbaspour H (2010) Nut Tress. Islamic Azad University Press. pp. 380. 28-223
Afshari H, Hokmabadi H (2008) Studying the Effects of Elements on Early Splitting of Pistachio Nuts andthe Effects of Phenolic Compounds on Aflatoxin Control. American-Eurasian Journal of Agriculture & Environment Science 4,131-137
Almasian S, Hoseini G, Sohani-Darban A, Farhad M (2008) Study of aflatoxin amount in corn meal. National Congress of Food Industry. 18,15-17
Amiri dumari H, Sarir H, Fani makki O, Afzali N (2013) Effects of Milk thistle seed against Aflatoxin B1 in broiler model. Journal of Research in Medical Sciences. 18 (9),786-790
Arrus K, Blank G, Abramson D, Clear R, Holley RA (2005) Aflatoxin production by Aspergillus flavus in Brazil nuts. Journal of Stored Products Research. 41(5),513-527. doi:
Berryman CE, Preston Ag Fau - Karmally W, Karmally W Fau - Deckelbaum RJ, Deckelbaum Rj Fau - Kris-Etherton PM, Kris-Etherton PM (2011) Effects of almond consumption on the reduction of LDL-cholesterol: a discussion of potential mechanisms and future research directions.  (1753-4887 (Electronic))
D'Mello JPF, Macdonald AMC (1997) Mycotoxins. Animal Feed Science and Technology. 69(1),155-166. doi:,81630-6.
Fani Makki O, Omidi A, Afzali N, Sarir H, Frouzanmehr M, Shibak A (2014) Efficacy of Silybum Marianum Seeds in Ameliorating the Toxic Effects of Aflatoxin B1 in Broilers. IJT. 8(24), 977-982
FAOSTAT (2012) Production Crops. Accessed 14 July.
Ghewande MP, Nagaraj G, Desai S, Narayan P (1993) Screening of groundnut bold seeded genotypes for resistance to Aspergillus flavus seed colonization and less aflatoxin production. Seed Science and Technology. 21, 45-51.
Gourama H, Bullerman LB (1995) Aspergillus flavus and Aspergillus parasiticus: Aflatoxigenic Fungi of Concern in Foods and Feeds: A Review. Journal of Food Protection. 58 (12), 1395-1404. Doi : 10 . 4315 / 0362 - 028 X - 58.12.1395
Gradziel TM, Wang D (1994) Susceptibility of california Almond cultivars to aflatoxigenic Aspergillus flavus. Hort Science.  29, 33-35
Jalali L, Afshari H, Ashraf S, Mohammadi Moghadam M (2011) Study on the sensitivity level of some pistachio cultivars of Khorasan-e-Razavi province to Aspergillus flavus. Journal of Nuts. 2,17-20
Mahoney ME, Rodrigues SB (1996) Aflatoxin variability in pistachios. Applied and Environmental Microbiology 62, 1197-1202.
Ricordy R, Gensabella G, Cacci E, Augusti-Tocco G (2002) Aflatoxin B1 and cell cycle perturbation. London:CRC Press. 4, 213-231
Trial F, Mahanti N, Linz J (1995) Molecular biology of aflatoxin biosynthesis. Microbiology. 141, 755-765.
Waliin JR (1986) Production of aflatoxin in wounded and whole maize kernels by Aspergillus flavus. Plant Diseases 10, 429-430.