Effects of Boron and Cold Stress on Germination of Almond Pollen in Vitro Culture

Document Type: Research Article

Authors

1 Department of Horticulture Science, University of Hormozgan, Hormozgan, Iran

2 Temperate Fruit Research Center of Horticultural Sciences Research Institute (HSRI), َAgricultural, Research, Edu-cation and Extension Organization, Tehran, Iran

Abstract

This study was carried out in order to determine the effects of boron and cold stress on pollen germination of almond hybrids in vitro culture, two different media including the absence and presence of boron (0 mg/l, and 100 mg/l) in 10% sucrose and 1% agar medium at 24°C in dark conditions. The results showed that different treatments had a significant effect on the germination percentage. The highest pollen germination percentage in H24 and S8 almond hybrids was recorded as 98.35 % and 97%, 34% in vitro culture medium containing 100 ppm boric acid, 10% sucrose +1% agar, whereas these amounts were 87.56% and 89.46% in the basic medium without the presence of boric acid, respectively. In conclusion, in vitro pollen germination of almond was inhibited in the medium culture without boron and in cold stress, while it was increased in the medium with boron. Thus, boron can increase the almond yield.

Keywords


 Acar I, Ak BE, Sarpkaya K (2010) Effects of boron and gibberellic acid on in vitro pollen germination of pistachio (Pistacia vera L.) cultivars. ‎ African Journal of Biotechnology.  9(32), 5126-5130.

Anderson JL, Seele Sdy (1993) Bloom delay in deciduous fruits. HorticultureReviews. 15, 97-144.

Ashworth EN, Anderson JA, Davis GA, Lightner GW (1985) Ice formationin Prunus persica under field conditions.  American Society for Horticultural Science.  110, 322–324.

Ballard JK, Proebsting EL, Tukey RB (1990) Critical temperatures for blossom buds of prunes, 374 (apricots), 913 (apples), 914 (peaches), 978 (pears), and 1128 (cherries). Coop Extension Bulletin 372 Washington State University, Pullman.

Brewbaker J, Majumder SK (1961) Cultural studies of pollen population effect and self-incompatibility inhibition. American Journal of Botany.48, 457-464.

Brown PH, Ferguson L, Picchioni G (1994) Boron nutrition of pistachio. California Pistachio Industry. Annual Report of Corporative Year. 1992-1993 60-63.

Charrier G, Ngao  J, Saudreau M,  Améglio T (2015) Effects of environmental factors and management practices on microclimate, winter physiology, and frost resistance in trees. Frontiers in PlantScience. 6, 259-266

Demotes-Mainard S, Doussinault G, Meynard JM (995) Effects of low radiation and low temperature at meiosis on pollen viability and grain set in wheat. Journal of Agronomy and Crop Science. 15(6), 357-365.

Dordas C, Brown PH (2000) Permeability of boric acid across lipid bilayers and factors affecting it. Journal of Membrane Biology. 175, 95-105.

Gupta M, Mutry YS (1985) In vitro hormonal regulation of pollen germination and tube growth of visia faba L. Biovigvanam. 112, 156-158.

Henny R (1977) Effect of sucrose level, medium composition and pH on the in vitro germination of pollen from Spathiphyllum floribundum (Linden Andre). N.E. Br. Mauna Loa and Vriesea Malzinei E. Morr. Proceedings of the Florida. State Horticultural Society. 90, 304-306.

Imani A, Barzegar K, Piri PS, Masomi SH (2011) Storage of apple pollen and in vitro germination. African Journal of Agricultural Research. 6(2), 624-629.

Khanal B (2010) Effect of day and night temperature on pollen Characteristics, fruit quality and storability of tomato. Master Thesis 60 credits, 2010-12.

Kester DE, Gradziel TM (1996) Almonds In: Fruit Breeding. Janick, J., Moore, J.N. (Eds) Vol. III. Nuts’. John Wiley and Sons: New York.

Loomis WD, Durst RW (1992) Boron and cell walls. Current topics in Plant Biochemistry and Physiology. 10, 149-178.

Martinez-Gomez P, Gradziel TM, Ortega E, Dicenta F(2002) Low temperature storage of almond pollen. American Society for Horticultural
Science. 37, 691-692.

Mussen EC, Montague MA (2004) Fungicide Impacts on almond pollen germination and tube elongation through the Pistils. Web site:entomology.ucdavis.edu/faculty/facpage.cfm?=mu.

Nyomora AMS, Brown PH (1997) Fall foliar-applied boron increases tissue boron concentration and nut set of almond. American Society for Horticultural Science. 122(3), 405-410.

Nyomora AMS, Brown PH, Pinney K, Polito VS (2000) Foliar application of boron to almond trees affects pollen quality. American Society for Horticultural Science. 125, 265– 270.

Proebsting EL (1985) Cold resistance of stone fruit flower buds.  PNW Coop Ext Bull, 221.

Rodrigo J (2000) Spring frosts in deciduous fruit trees morphological damage and flower hardiness. Scientia Horticulturae. 85, 155–173.

SAS  (1996) User’s Guide, Release 6.13, SAS Institute Inc., cary, NC,USA.

Shivanna KR, Linskens HF, Cresti M(1991) Pollen viability and pollen vigor. ‎Theoretical and Applied Genetics. 81, 38-42.

Socias I company R (1992) Breeding Self-fertile
almonds. Plant Breeding Reviews. 8, 313-38.

Stanley RG, Linskens HF (1974) Pollen Biology Biochemistry Management. Springer-Verlag, NewYork, pp. 307.

Tosun F, Koyuncu F (2007) Effects of some chemical treatments on pollen germination and pollen tube growth in sweet cherries (Prunus avium L.). Ziraat Fakültesi Dergisi (Akdeniz Üniversitesi- Meditrranean Agricultural Sciences). 20(2), 219-224.

Vitagliano C, Viti R (1989) Effects of some growth substances on pollen germination and tube growth in different stone fruits. Acta  Horticulturae. 239,379-381.

Wang QL, Lu LD, Wu XQ, Li YQ, Lin JX (2003) Boron influences pollen germination and pollen tube growth in Picea meyeri. Tree Physiology. 23, 345–351.