The Effects of Humic Acid and Calcium on Morpho-Physiological Traits and Mineral Nutrient Uptake of Pistachio Seedling under Salinity Stress

Document Type : Research Article


1 Pistachio Research Center, Horticulture Sciences Research Institute, Agriculture Research Education and Extension Organization (AREEO), Rafsanjan, Iran

2 Department of Plant Science, Payame Nour University of Esfahan, Esfahan, Iran


The study was carried out to evaluate the effects of soil application of humic acid and calciumon morpho-physiological traits and Na+, Mg2+, Ca2+ uptake of Pistachio seedling (Akbari) grown under salt stress.  A completely randomized design was used with four replications in greenhouse conditions. The experimental treatment consisted of four levels of humic acid (Bis humic) (0, 4, 8 and 12 gr kg-1 soil) and anti-salt as a source of Ca+2 (0, 1, 2 and 3 gr kg-1 soil). Results indicated that humic acid and  anti-salt significantly increased vegetative growth, such as root fresh and dry weight, stem diameter, leaf number and leaf area, of pistachio seedling in comparison with the control group (p<0.05). However, no significant differences in fresh and dry weight of shoot of pistachio seedling were detected in all treatments except in humic acid 8 gr kg-1 soils. The results indicated that the application of humic acid and anti-salt decreased Na+ absorption in pistachio seedling in all experimental treatments (p<0.05). No significant differences in Ca+2 and Mg+2 absorption were detected in all treatments. Results showed a significant reduction of the electrical conductivity (EC) and sodium adsorption ratio (SAR) in all levels of humic acid and anti-salt in soil.  However, in these treatments, there were no statistically significant differences in the pH. The best concentration of humic acid and anti-salt was 8 gr kg-1 soil and 3 gr kg-1 soil, respectively. This study revealed the relative importance and efficiency of humic acid and anti-salt to salinity stress tolerance in pistachio seedling.


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