The Interaction Effects of Boron and Plant Growth Regulators on Pollen Germination of Almond

Document Type : Research Article


1 Department of Horticulture, Agriculture-Jihad Organization, Qazvin, Iran

2 Horticultural Department of Seed and Plant Improvement Institute (SPII), Karaj, Iran

3 Center of brilliant talents Shahid Soltani, Karaj, Iran


The aim of this study was to determine the effects of different plant growth regulators, NAA (0 mg/l, 50 mg/l and 100 mg/l) and GA3 (0 mg/l, 50 mg/l and 100 mg/l), with various boron concentrations (0 mg/l, 50 mg/l and 100 mg/l) on pollen germination of ‘Şaba’, ‘Rabie’ and ‘Padre’ almond cultivars in 10% sucrose and 1% agar medium at 24°C in dark conditions. The results showed that different treatments had significant effects on the percent of germination. The highest pollen germination (average 90.37%) for three almond cultivars was recorded in 100 mg/l boric acid, 10% sucrose and 1% agar medium. The lowest germination percentage (average 3.59%) was found in 50 mg/l NAA, sucrose 10 % and agar 1 % medium for all cultivars. Pollen germination rates significantly decreased with increasing growth regulators in almond cultivars. The pollen germination was greatly inhibited in media with GA3 and NAA or compound from these materials without boric acid. For example, mean pollen germination rates was %15.51 in 100 mg/l GA3+ 10% sucrose + 1% agar, while this value was 82.89% from the 100 mg/l GA3+100 mg/l Br+ 10% sucrose + 1% agar.


Acar I, Ak BE, Sarpkaya K (2010) Effects of boron and gibberellic acid on in vitro pollen germination of pistachio (Pistacia vera L.) cultivars. African Journal of Biotechnology. 9 (32), 5126-5130.
Bhandal IS, Malik CP (1979) Effect of gibberellic acid, (2-chloroethyl) phosphoric acid, actinomycin-D and cycloheximide on the activity and leaching of some hydrolases in pollen suspension cultures of Crotalaria juncea. Physiology of Plant. 45, 297-300.
Bolat I, Pirlak L, Karayannis I (1999) Effects of some chemical substances on pollen germination and tube growth in apricot. Acta Horticulturae. 488(1), 341-344.
Brewbaker J, Majumder SK (1961) Cultural studies of pollen population effect and self-incompatibility inhibition. American Journal of Botany. 48, 457-464.
Dordas C, Brown PH (2000) Permeability of boric acid across lipid bilayers and factors affecting it. Journal of Membrane Biology. 175, 95–105.
Gupta M, Mutry YS (1985) In vitro hormonal regulation of pollen germination and tube growth of visia faba L. Biovigvanam. 112, 156-158.
Henny R (1977) Effect of sucrose level, medium composition and pH on the in vitro germination of pollen from Spathiphyllum floribundum (Linden Andre). N.E. Br. Mauna Loa and Vriesea Malzinei E. Morr. Proceedings of the Florida State Horticultural Society. 90, 304-306.
Imani A, Barzegar K, Piri PS, Masomi SH (2011) Storage of apple pollen and in vitro germination. African Journal of Agricultural
Research. 6(2), 624-629.
Kester DE, Gradziel TM (1996) Almonds In: Fruit Breeding. Janick J, Moore JN, (Eds). Vol. III. Nuts’. John Wiley and Sons: New York.
Korkutal I, Bahar E, Gökhan Ö (2008) The characteristics of substances regulating growth and development of plants and the utilization of gibberellic acid (GA3) in viticulture. World Journal of Agricultural Sciences. 4(3), 321-325.
Loomis WD, Durst RW (1991) Boron and cell walls. Current Topics in Plant Biochemistry and Physiology. 10, 149–178.
Martinez-Gomez P, Gradziel TM, Ortega E, Dicenta F (2002) Low temperature storage of almond pollen . American Society for Horticultural Science. 37, 691-692.
Mussen EC, Montague, MA (2004) Fungicide Impacts on Almond Pollen Germination and Tube Elongation Through the Pistils. Web
Nyomora AMS, Brown PH (1997) Fall foliar-applied boron increases tissue boron concentration and nut set of almond. American Society for Horticultural Science. 122(3), 405-410.
Nyomora AMS, Brown PH, Pinney K, Polito VS (2000) Foliar application of boron to almond trees affects pollen quality. American Society for Horticultural Science. 125, 265– 270.
SAS (1996) User’s Guide, Release 6.13, SAS Institute Inc., cary, NC, USA.
Setia N, Setia RC, Chabra N (1994) Interactive effects of growth hormones and calcium antagonists on germination and tube elongation of groundnut pollen. Plant Cell Incompatibility Newsletter. 26, 70-80.
Shivanna KR, Linskens HF, Cresti M (1991) Pollen viability and pollen vigor. Theoretical
Applied Genetics. 81, 38-42.
Socias I company R (1992) Breeding Self-fertile almonds. Plant Breeding Reviews. 8, 313-338.
Stanley RG, Linskens HF (1974) Pollen: Biology, Biochemistry, Management. Springer-Verlag, New York.
Swamy AVSR, Khanna VK (1991) Effect of some growth regulators on in vitro pollen germination and tube growth in Cicer. International of Chickpea Newsletter. 25, 32-34.
Tosun F, Koyuncu F (2007) Effects of some chemical treatments on pollen germination and pollen tube growth in sweet cherries (Prunus avium L.). Akdeniz University Ziraat Fakuty Dergisi. 20(2), 219-224.
Vitagliano C, Viti R (1989) Effects of some growth substances on pollen germination and tube growth in different stone fruits. Acta Horticulturae. 239, 379-381.
Viti R, Bartolini S, Vitagliano C (1990) Growth regulators on pollen germination in olive. Acta Horticulturae. 286, 227-230.
Wang QL, Lu LD, Wu XQ, Li YQ, Lin JX (2003). Boron influences pollen germination and pollen tube growth in Picea meyeri. Tree Physiology. 23, 345–351.