Identification and Screening of Homozygous and Heterozygous Almond Progenies from Self-Pollinated Touno Cultivar Using PCR

Authors

1 Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Department of Horticultural, Seed and Plant Improvement Iinstitute (SPII), Karaj, Iran

3 Department of Molecular Genomics, Agricultural Biotechnology Research Institute (ABRII), Karaj, Iran

Abstract

Self-incompatibility is one of the most important difficulties in almond production which reduce fruit set dramatically and makes orchard management difficult. Therefore, breeding almond to produce self-compatible genotypes is very important. In this research identification and screening of 86 almond progenies obtained from selfing Touno after the self-pollination by PCR reaction with specific primers of CEBASf and AS1. PCR results confirmed the situation of self-compatible hybrids. In addition, it indicated that, frequencies of Sf, and S1 was 100% and 50% in progenies respectively. Self-compatible hybrids had been identified that can be used in almond breeding programs particularly to development the monoculture of almond orchards. So to identify and screening homozygous self-compatibility almonds be capable of be another step towards creating monoculture of almond and use in breeding programs further.

Keywords

Journal of Nuts

Volume 06, Issue 02
Summer and Autumn 2015
Pages 155-164

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