Shahmoradi, M., Rasouli, M., Footuhi Ghazvini, R., Imani, A., Hamidogli, Y. (2013). Discrimination and preliminary selection of self-compatible progenies among controlled crosses in almond using Sf specific primer. Journal of Nuts, 04(01,02), 47-54. doi: 10.22034/jon.2013.515714
M. Shahmoradi; M. Rasouli; R. Footuhi Ghazvini; A. Imani; Y. Hamidogli. "Discrimination and preliminary selection of self-compatible progenies among controlled crosses in almond using Sf specific primer". Journal of Nuts, 04, 01,02, 2013, 47-54. doi: 10.22034/jon.2013.515714
Shahmoradi, M., Rasouli, M., Footuhi Ghazvini, R., Imani, A., Hamidogli, Y. (2013). 'Discrimination and preliminary selection of self-compatible progenies among controlled crosses in almond using Sf specific primer', Journal of Nuts, 04(01,02), pp. 47-54. doi: 10.22034/jon.2013.515714
Shahmoradi, M., Rasouli, M., Footuhi Ghazvini, R., Imani, A., Hamidogli, Y. Discrimination and preliminary selection of self-compatible progenies among controlled crosses in almond using Sf specific primer. Journal of Nuts, 2013; 04(01,02): 47-54. doi: 10.22034/jon.2013.515714
Discrimination and preliminary selection of self-compatible progenies among controlled crosses in almond using Sf specific primer
1Department of Horticultural science, Faculty of Agriculture, Guilan University, Rasht, Iran
2Department of Horticulture, Faculty of Agriculture, Malayer University, Malayer, Iran
3Department of Horticultural, Seed and Plant Improvement Institute (SPII), Karaj 31587, Iran
Abstract
Self-incompatibility in almond and Prunus species is an important trait that prevents self-fertilization. Selfincompatibility in almond is controlled gametophytically by the multiallelic S-locus. Present study was done in order to identification and preliminary selection of self compatible progenies resulted from controlled crosses in almond using specific primer SfF/SfR. Some important morphological traits of parental crosses were evaluated using almond descriptor. Also, progenies (F1) of five crosses inculding; A (Tuono × 101 Genotype), B (Supernova × 101 Genotype), C (Genco × Shahrood 21), D (Tuono × Shahrood 12) and E (Tuono × shahrood 17) were tested using PCR in order to DNA amplification and self-compatibility evaluation. The result of PCR method found that using SfF/SfR pair primer, self-compatible progenies showed 449 bp bands, while this band not observed in self-incompatible progenies. In addition, all self- incompatible progenies appeared no S1 allele at any condition. According to Chi-square test, ratios of self-compatible to self-incompatible progenies were to Mendelian principles. It can be suggested that PCR method of this research has high potential in order to distinguish self-compatible and incompatible genotypes.
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